Shaggy ink cap - Coprinus comatus (O.F. Mull.) has been known worldwide due to its great medicinal and nutritional values. The aim of this study was to acquire basic data regarding the suitable conditions for C. comatus mycelium growth in culture medium. The results of the study illustrated that PDPYA and YEPD were the most suitable culture media for cultivation of C. comatus. The optimal temperature, pH, and the length of lightening time for the mycelium growth of C. comatus master spawn were 25±1o C, pH = 6-7, and 0 h, respectively. The pH, temperature, carbon and nitrogen sources for optimal growth of liquid spawn in submerged medium were pH = 7.0; 25±1o C; sucrose and peptone, yeast extract, respectively.